Table 2 Characteristics of Pseudomonas fluorescens OS8-based plasmid-containing luminescent heavy metal sensors and toxicity control strains
| Â | Sensor strains | Control strain | ||
|---|---|---|---|---|
| Â | OS8(pDNmerRBSB Pmerlux) | OS8(pDNpbrR PpbrAlux) | OS8(pDNcadR PcadAlux) | OS8(pDNlux) |
Background luminescence a | 1600 ± 540 | 3960 ± 1300 | 25900 ± 8700 | 64500 ± 6600 |
Maximum induction b | 200 | 7 | 6 | no induction |
No of cells in the test (100 μl) | 5 × 106 | 4 × 106 | 4 × 106 | 5 × 106 |
Compound | Limit of determinationc/Toxicityd, M (mg of compound l-1) | Toxicity (2-h EC50e), M (mg of compound l-1) | ||
HgCl 2 | 10-9 (0.0003)/10-7 (0.04) | 10-7 (0.04)/10-6 (0.4) | 10-7 (0.03)/10-6 (0.4) | 2.5 × 10-7 (0.07) |
CH 3 HgCl | 10-10 (0.00003)/10-7 (0.04) | not tested | not tested | 2 × 10-6 (0.5) |
CdCl 2 | 5 × 10-6 (0.9)/10-2 (2740) | 8 × 10-7 (0.2)/5 × 10-4 (92) | 6 × 10-8 (0.01)/10-4 (18) | 4 × 10-2 (7300) |
ZnSO 4 f | No induction | 4 × 10-5 (6.4)/5 × 10-2 (8050) | 8 × 10-6 (1.3)/3 × 10-2 (4800) | 3 × 10-2 (4800) |
Pb(NO 3 ) 2 | No induction | 9 × 10-7 (0.3)/10-3 (500) | 4 × 10-7 (0.1)/10-3 (500) | 7 × 10-4 (230) |
CuSO 4 g | not tested | not tested | not tested | not tested |
AgNO 3 | not tested | not tested | not tested | not tested |