A modified and automated version of the 'Fluorimetric Detection of Alkaline DNA Unwinding' method to quantify formation and repair of DNA strand breaks

Table 1 Influence of cell titre on fluorescence signal intensity.

Cells/μl	Ratios of fluorescence signal intensities			Standard deviation [%]
	P₀/T	P₁/P₀	P₂/P₁	T	P₀	P₁	P₂
297	0.82	0.94	0.77	5.5	10.6	12	12.7
132	0.84	0.96	0.68	3.3	4.5	4.9	5.4
90	0.86	0.95	0.71	2.9	4.6	4.2	3.2

P₁ and P₂ samples were irradiated with 0.47 or 2.3 Gy, respectively. Standard deviation was evaluated for 8 replicates, respectively, and expressed as % of mean values.

ISSN: 1472-6750