Figure 6
From: Acetylation of PAMAM dendrimers for cellular delivery of siRNA
Confocal microscopy images of Cy-5 labeled oligonucleotides delivered to U-87-d1EGFP cells. U-87-d1EGFP cells were treated with polymer/Cy5-ODN complexes (N/P = 15). Images show U-87-d1EGFP cells in green, and Cy5-ODN in red. Images represent cells transfected with: (a) G5 PAMAM; (b) Ac60 PAMAM; (c) 25K PEI; (d) OptiMEM medium only; (e) G5 PAMAM + 100 μM chloroquine; (f) Ac60 PAMAM + 100 μM chloroquine; (g) 25K PEI + 100 μM chloroquine; and (h) Lipofectamine2000.