Figure 6
From: Gene Composer: database software for protein construct design, codon engineering, and gene synthesis
Synonymous introduction and removal of DNA restriction enzyme sites. The Protein-to-DNA algorithm of Gene Composer has generated a novel back-translated gene sequence, and has identified existing restriction sites that either can be eliminated or introduced (listed above or below the nucleic acid sequence respectively) by synonymous mutation without violating any user defined constraints on codon usage or G:C% content. Unique, single cutting restriction sites are shown in green. The inset window shows a detailed list the location of each restriction site, its current sequence (from: column), and the proposed alternate synonymous sequence (to: column) required to silently introduce/remove the restriction site.