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Figure 5 | BMC Biotechnology

Figure 5

From: Optimization of codon composition and regulatory elements for expression of human insulin like growth factor-1 in transgenic chloroplasts and evaluation of structural identity and function

Figure 5

Northern blot analysis. A) The map of pLD-5'UTR-ZZTEVIGF-1 shows a monocistron transcript of 1099 nt, a dicistron transcript of 2019 nt, and polyciston transcript of 4519 nt. B) Lane 1: untransformed control. Lanes 2–3: 5'UTRZZTEVIGF-1s transgenic lines (T0); Lanes 4–7: 5'UTRZZTEVIGF-1s transgenic lines (T1); Lanes 8–9 5'UTRZZTEVIGF-1n Protein quantification by ELISA in (T0); Lane 10: blank; Lane 11: IGF-1 probe used as a positive control.

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BMC Biotechnology

ISSN: 1472-6750

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