Figure 3

Analysis of H2AX phosphorylation using co-transfection of plasmids encoding EGFP and PhiC31 integrase or Sleeping Beauty (SB) transposase. a) Cells co-transfected with plasmids encoding EGFP (green) and integrase were immunostained using anti-γ-H2AX antibodies (red). b) H2AX immunostaining was quantified by ranking H2AX positive cells from 1 to 3, 3 being the very intensive nuclear staining and 1 being the vague but yet defined nuclear staining. Staining intensities were quantified in GFP positive cells.