Figure 1
From: Simultaneous detection of mRNA and protein stem cell markers in live cells
Changes of Oct-4 mRNA and protein levels 4 days after retinoic acid (RA) treatment in P19 embryonal carcinoma cells. A. Real-time PCR result of Oct-4 mRNA level in undifferentiated (UD) and differentiated (RA treated) P19 cells. B. Immunocytochemistry of Oct-4 protein (Red) before (UD) and after (RA) differentiation. Cell nuclei were stained with Hoechst 33342 (Blue).