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Figure 3 | BMC Biotechnology

Figure 3

From: Folding machineries displayed on a cation-exchanger for the concerted refolding of cysteine- or proline-rich proteins

Figure 3

Functional analysis of immobilized folding machineries, DsbA and hPPIase. DsbA (A) and hPPIase (B) assay were based on their enzymatic properties to catalyze protein disulfide bond formation and cis-trans interconversion of the peptidyl-prolyl bond, respectively. RNase A with scrambled disulfide bonds and N-Suc-AAPF-p-nitroanilide were used for determination of activities of immobilized DsbA and hPPIase as substrate as described in Methods section.

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BMC Biotechnology

ISSN: 1472-6750

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