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Table 3 Comparative time frames of reported panning protocols

From: Chromato-panning: an efficient new mode of identifying suitable ligands from phage display libraries

Microtiter plate [28] Microtiter plate [29] RISE [30]
Round 1 (h) Round 1 (h) Round 1 (h)
Target incubation 18 Target incubation 2 Target incubation 18
Wash-postcoat 2.25 Wash-postcoat 1.5 Wash-postcoat 4
Phage incubation 1 Phage incubation 1 Cell growth tubes 3
Wash-elution 0.5 Wash-elution 2.25 Infection 3
Infection   Infection   Wash 0.5
- amplification 4.5 - amplification 5 Cell/phage/target incubation 16
Phage recovery 0.5 Phage recovery 18 Phage recovery 0.5
Time ~27 h   ~30 h   ~45 h
2 additional panning rounds   2 additional panning rounds   3 additional panning rounds  
TOTAL TIME ~81 h   ~90 h   ~180 h
  1. A mean time frame of each step in the panning procedure is presented for one panning round. The total time represents the time frame for a complete panning procedure as reported in the literature.