Table 3 Comparative time frames of reported panning protocols
From: Chromato-panning: an efficient new mode of identifying suitable ligands from phage display libraries
Microtiter plate [28] | Microtiter plate [29] | RISE [30] | |||
|---|---|---|---|---|---|
Round 1 (h) | Round 1 (h) | Round 1 (h) | |||
Target incubation | 18 | Target incubation | 2 | Target incubation | 18 |
Wash-postcoat | 2.25 | Wash-postcoat | 1.5 | Wash-postcoat | 4 |
Phage incubation | 1 | Phage incubation | 1 | Cell growth tubes | 3 |
Wash-elution | 0.5 | Wash-elution | 2.25 | Infection | 3 |
Infection | Infection | Wash | 0.5 | ||
- amplification | 4.5 | - amplification | 5 | Cell/phage/target incubation | 16 |
Phage recovery | 0.5 | Phage recovery | 18 | Phage recovery | 0.5 |
Time | ~27 h | ~30 h | ~45 h | ||
2 additional panning rounds | 2 additional panning rounds | 3 additional panning rounds | |||
TOTAL TIME | ~81 h | ~90 h | ~180 h | ||