Table 2 Comparative time frame of chromato-panning protocol and tube panning protocol
From: Chromato-panning: an efficient new mode of identifying suitable ligands from phage display libraries
Chromato-panning | Tube panning | ||
|---|---|---|---|
Round 1 (h) | Round 1 (h) | ||
Column equilibration | 0.25 | Target coating | 18 |
Wash-postcoat | 2.25 | ||
On column panning | 1 | Phage incubation | 20 |
Washing/elution | 0.50 | ||
On column infection | 0.50 | Infection | 0.50 |
Elution | 0.25 | ||
Plating/amplification | 18 | Plating/amplification | 18 |
Phage recovery | 3 | Phage recovery | 3 |
Time | ~23 h | ~62 h | |
No additional panning rounds are needed | 2 additional panning rounds are performed Phage incubation R2/3 | 2.50 | |
TOTAL TIME | ~23 h | ~152 h | |