Chromato-panning: an efficient new mode of identifying suitable ligands from phage display libraries

BMC Biotechnology

Table 1 Statistical analysis data

Library	Id	Panning round	Top-value	95% CI	R²
C6	a	T r1	0.853	[0.712–0.994]^bc	0.979
	b	T r3	1.622	[1.382–1.862]^a	0.978
	c	C r1	1.605	[1.370–1.839]^a	0.981
L6	a	T r1	0.697	[0.600–0.794]^bc	0.990
	b	T r3	1.640	[1.500–1.780]^a	0.990
	c	C r1	1.545	[1.299–1.790]^a	0.962
L15	a	T r1	1.354	[1.279–1.430]^c	0.999
	b	T r3	1.382	[1.180–1.584]^c	0.986
	c	C r1	1.832	[1.645–2.020]^ab	0.994

For the three phage libraries C6, L6 and L15, top-values, 95% CI and R² (see Material and Methods) was calculated and compared for the tube panning round 1, 3 (Tr1, Tr3) and chromato-panning procedure round 3 (Cr1). Superscripts denote statistical significant difference (p < 0.05) between top-values (e.g. top "a" is statistically significant different from tops "b" and "c").

ISSN: 1472-6750