Figure 1From: An optimised system for refolding of human glucose 6-phosphate dehydrogenaseEffects of different concentrations of NADP+ on the refolding recovery yield. NADP+ was added into the refolding buffer containing 50 mM NaCl, 400 mM Arg, 10 mM DTT, and 40 μM PEG 3350 in 50 mM Tris, pH 8.0 to reach final NADP+ concentrations from 0 to 400 μM. The refolding mixture was incubated at 25°C and the final regained G6PD activity was followed.Back to article page