Figure 2From: Improving the functional expression of a Bacillus licheniformislaccase by random and site-directed mutagenesisSDS-PAGE analysis of constructed mutants and wild-type CotA. Whole cells and cell lysates of the CotA mutants D500G (D), K316N (K), and K316N/D500G (DK) as well as of wild-type CotA (CotA) expressed overnight in E. coli BL21(DE3) cells; molecular size marker from Fermentas (M).Back to article page