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Table 4 Recovery of lentiviral vectors following Mustang Q anion exchange chromatography and buffer exchange

From: Simplified production and concentration of HIV-1-based lentiviral vectors using HYPERFlask vessels and anion exchange membrane chromatography

Input (TU) Unbound (TU) Bound (TU) Recovered following Mustang Q chromatography (TU) Recovered following buffer exchange (TU)
7.3 ± 2.0 × 1010 7.4 ± 2.3 × 109 6.5 ± 1.8 × 1010 4.1 ± 0.6 × 1010 4.5 ± 0.8 × 1010
  1. Mustang Q Acrodiscs were challenged with 500-ml aliquots of HYPERFlask vessel-derived vector supernatants. Buffer exchange was carried out by Sepharose CL-4B size exclusion chromatography as outlined under Materials and Methods. Titers were determined by FACS using HOS cells. Data from three independent productions are shown. Data are expressed as mean ± SD.