Figure 3From: Reporter gene-expressing bone marrow-derived stromal cells are immune-tolerated following implantation in the central nervous system of syngeneic immunocompetent miceSurvival of BMSC genetically modified with multiple reporter genes following implantation in the central nervous system of syngeneic immunocompetent mice. (A) Histogram overlay showing a representative flow cytometric analysis of eGFP expression by BMSC expressing the luciferase-, eGFP- and puromycin resistance genes (BMSC-Luc/eGFP/Pac, filled histogram). Parental BMSC were used as negative control (open histogram). (B) In vitro luminescence assay on parental BMSC and on clonal BMSC-Luc/eGFP/Pac. (C) In vivo real time bioluminescence imaging (BLI) of clonal BMSC-Luc/eGFP/Pac grafts in the central nervous system (CNS) of syngeneic immunocompetent mice at week 2 post-implantation. (D) In vivo real time BLI of clonal BMSC-Luc/eGFP/Pac intramuscular grafts in syngeneic immunocompetent mice at week 2 post-injection. (E) Representative histological analysis of clonal BMSC-Luc/eGFP/Pac implants in the CNS of syngeneic immunocompetent mice at week 2 post-implantation. Left picture: haematoxylin-eosin (HE) staining showing general appearance of the cell implantation site. Middle picture: direct eGFP-fluorescence indicating the BMSC-Luc/eGFP/Pac origin of the observed cell implant. Right picture: CD11b staining indicating a limited number of microglia surrounding the observed cell graft. All slides were examined using a conventional bright field microscope and digital pictures were taken under magnification as indicated by the scale bars.Back to article page