| Average scFv Rankings | Major Germline Families | ||||
---|---|---|---|---|---|---|
Clone | Supernatant | Periplasm | Spheroplast | Display | VH | VL |
C1 | 6.0 ± 1 | 9.8 ± 0.8 | 7.0 ± 1.2 | 11.7 ± 1.1 | VH4 | VL3 |
C2 | 6.0 ± 0 | 14.3 ± 0.7 | 3.0 ± 0.6 | 6.2 ± 0.5 | VH1 | VL3 |
C3 | 7.5 ± 0.5 | 3.5 ± 0.5 | 8.7 ± 2.9 | 6.3 ± 1.0 | VH4 | VK1 |
C4 | 14.5 ± 0 | 11.8 ± 1.2 | 4.0 ± 1.5 | 4.2 ± 1.2 | VH4 | VK1 |
C5 | 14.5 ± 0 | 6.0 ± 1 | 3.7 ± 1.5 | 2.7 ± 0.3 | VH4 | VK1 |
N1 | 10.0 ± 2 | 13.8 ± 3.5 | 10.0 ± 0.6 | 7.5 ± 2.2 | VH2 | VK1 |
N2 | 4.0 ± 0 | 4.5 ± 2.2 | 3.3 ± 1.9 | 2.8 ± 1.1 | VH1 | VK1 |
N3 | 13.0 ± 0 | 10.8 ± 0.5 | 12.0 ± 0 | 11.5 ± 0.9 | VH3 | VK1 |
N4 | 9.0 ± 0 | 3.5 ± 0.5 | 9.3 ± 2.0 | 12.2 ± 1.1 | VH3 | VL2 |
N5 | 8.3 ± 3.3 | 4.3 ± 2.7 | 11.0 ± 1.0 | 10.7 ± 1.6 | VH3 | VL1 |
J1 | 10.5 ± 0.5 | 9.0 ± 3 | 14.7 ± 0.3 | 12.2 ± 1.1 | VH3 | VL2 |
J2 | 2.0 ± 0 | 11.8 ± 1.8 | 4.0 ± 1.5 | 3.8 ± 0.5 | VH1 | VK1 |
J3 | 2.0 ± 1 | 3.8 ± 2.2 | 5.3 ± 1.3 | 6.5 ± 1.3 | VH3 | VL2 |
J4 | 2.0 ± 1 | 3.5 ± 1.5 | 10.0 ± 1.0 | 11.3 ± 0.7 | VH6 | VL2 |
J5 | 10.8 ± 0.7 | 10.0 ± 1 | 14.0 ± 0.6 | 11.3 ± 1.5 | VH3 | VL2 |