| |
Average scFv Rankings
|
Major Germline Families
|
|---|
|
Clone
|
Supernatant
|
Periplasm
|
Spheroplast
|
Display
|
VH
|
VL
|
|---|
|
C1
|
6.0 ± 1
|
9.8 ± 0.8
|
7.0 ± 1.2
|
11.7 ± 1.1
|
VH4
|
VL3
|
|
C2
|
6.0 ± 0
|
14.3 ± 0.7
|
3.0 ± 0.6
|
6.2 ± 0.5
|
VH1
|
VL3
|
|
C3
|
7.5 ± 0.5
|
3.5 ± 0.5
|
8.7 ± 2.9
|
6.3 ± 1.0
|
VH4
|
VK1
|
|
C4
|
14.5 ± 0
|
11.8 ± 1.2
|
4.0 ± 1.5
|
4.2 ± 1.2
|
VH4
|
VK1
|
|
C5
|
14.5 ± 0
|
6.0 ± 1
|
3.7 ± 1.5
|
2.7 ± 0.3
|
VH4
|
VK1
|
|
N1
|
10.0 ± 2
|
13.8 ± 3.5
|
10.0 ± 0.6
|
7.5 ± 2.2
|
VH2
|
VK1
|
|
N2
|
4.0 ± 0
|
4.5 ± 2.2
|
3.3 ± 1.9
|
2.8 ± 1.1
|
VH1
|
VK1
|
|
N3
|
13.0 ± 0
|
10.8 ± 0.5
|
12.0 ± 0
|
11.5 ± 0.9
|
VH3
|
VK1
|
|
N4
|
9.0 ± 0
|
3.5 ± 0.5
|
9.3 ± 2.0
|
12.2 ± 1.1
|
VH3
|
VL2
|
|
N5
|
8.3 ± 3.3
|
4.3 ± 2.7
|
11.0 ± 1.0
|
10.7 ± 1.6
|
VH3
|
VL1
|
|
J1
|
10.5 ± 0.5
|
9.0 ± 3
|
14.7 ± 0.3
|
12.2 ± 1.1
|
VH3
|
VL2
|
|
J2
|
2.0 ± 0
|
11.8 ± 1.8
|
4.0 ± 1.5
|
3.8 ± 0.5
|
VH1
|
VK1
|
|
J3
|
2.0 ± 1
|
3.8 ± 2.2
|
5.3 ± 1.3
|
6.5 ± 1.3
|
VH3
|
VL2
|
|
J4
|
2.0 ± 1
|
3.5 ± 1.5
|
10.0 ± 1.0
|
11.3 ± 0.7
|
VH6
|
VL2
|
|
J5
|
10.8 ± 0.7
|
10.0 ± 1
|
14.0 ± 0.6
|
11.3 ± 1.5
|
VH3
|
VL2
|
- Densitometry data derived from the western blots shown in Figures 1 and 2 were used to rank scFvs from 1 to 15 in order of highest to lowest levels of scFv respectively. Rankings from repeat experiments were averaged and are shown here along with standard error for the overall expression and display levels. The average relative spheroplast scFv level rankings were used as an indicator of overall expression level and to designate the scFvs into three groups of either "high (average ranking ≤ 5; bold numbers)", "medium (average ranking ≥ 5 but ≤ 10; italicised numbers) " or "low" (average ranking ≥ 11; No Shading) expression. Rankings for phage display propensity were also used to assign similar groupings. The major VH and VL germline designations for each scFv are also shown for each clone.
