Table 1 Oligonucleotide primers used in this study
Primer name | Sequence 5'-3' |
|---|---|
Tc-5'-PmlNco | CACGTGCCATGGTTCTCATGTTTGACAGCTTATC |
Tc-3'-PmlNsi | CACGTGAT GCATGGACTTCCGCGTTTCCAG |
SpR-5'-HpaBspNhe | ATCGTTAACTCATGAGCTAGCCGTTCGTGAATACATGTTATAATA |
SpR-3'-Hpa | ATCGTTAACTTATTTGCCGACTACCTTGGTGATCT |
GFP-mu-5'-Bam | ATCGGATCCAAAACAATGGCTAGTAAAGGAGAAGAACTTTTCACTGGAGTTGTCCC |
GFP-mu-3'-NotAvrBgl | GATATCGCGGCCGCCCTAGGTAGATCTTCCACCTCCACCTTTGTATAGTTCATCCATGCCATGTGTA |
ABI3-5'-CDS-Rescue-3'-Test | CTCCTCCATGGTCATCTAACC |
ABI3-3'-CDS-Rescue-5'-Test | GTACCCGAGTGTGCCACTTC |
PAP85-Prom-Rescue-3'-Test | ACTTCCTAGGGTTGCTACTG |
PAP85-Term-Rescue-5'-Test | ACCGGATGGTTTGGT TATGG |
ABI3-L1-Rescue-5'-CDS1 | agatctccggcgttggccgccacatgcaagcttttcatcgttgaagtggaCAAATACGCATACTGTTATCTGGCT |
ABI3-L2-Rescue-3'-CDS1 | tattacatatgtggctcatttcttgagtcgagcttacatatcgtttataaAACCTGATGTTCTGGGGAATATAG |
ENTRY-L1 | TTAGTTAGTTACTTAAGCTCGG |
ENTRY-L2 | TGAGACACGGGCCAGAGC |
PAP85-Rescue-5'-Prom2 | ttcctaccaccatgaccaaacttggaatcccgaaactccctcttcttgttTTGTCGTTTCCCGCCTTCAG |
PAP85-Rescue-3'-Term2 | atagctcgtattttttagcttatgtctaaaatacccctgagatcatgtggAACGTTCTCGAGGGGATCTTCTG |
pCB-RB | CTGTGGTTGGCATGCACATA |
tCUP2-Out | CGAGGTTAGACCTTCAGGAAATA |
PAP85-Integ-Dest-5'3 | acccattattgtcctcaaaaacaaacacatcaacaaaacaacaagaaaaaGATATCACAAGTTTGTACAAAAAAGC |
PAP85-Integ-Dest-3'3 | ttaaactcttaaactctttcctttcttaggactgtaaaaaagcattttcaGATATCACCACTTTGTACAAGAAAGC |
PAP85-Dest-Integ-5'-Test | TGATGCGTACGAACACTC |
Cm-5'-AS | ACGGTGGTATATCCAGTGATT |
PAP85-Dest-Integ-3'-Test | GTTACATGCAGAGCG TTACC |
C1 | TTATACGCAAGGCGACAAGG |
PAP85-S1 | GCAGGGATCCT TGTTCCTTC |