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Table 2 Characterization of clones producing neutralizing IgG to HCMV

From: Generation of potent neutralizing human monoclonal antibodies against cytomegalovirus infection from immune B cells

 

10B7

1F7

26A1

8C10

Donor

CMV5

CMV5

CMV7

CMV7

Doubling Time (days)

4

4

4

4

IgG Subclass

IgG1

IgG1

IgG1

IgG2

BEIA-CMV a

-

-

+

+

gB ELISA

+

-

-

+

gH ELISA

-

+

-

-

HELF binding b

-

-

-

-

HUVEC binding b

-

-

-

-

HCMV neutralization c

64.72 ± 11.34

47.23 ± 2.98

86.34 ± 8.15

52.3 ± 13.34

  1. a. Antibodies were tested using the BEIA CMV ELISA assay
  2. b. Antibodies were tested for binding to uninfected HELF and HUVEC by immunohistochemistry.
  3. c. Antibodies were tested using the HCMV microneutralization assay, with the HCMV AD169 strain and HELF. Results are expressed as % of inhibition of virus infectivity. Data represent the mean ± s.d. of 3–4 experiments.