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Table 2 Characterization of clones producing neutralizing IgG to HCMV

From: Generation of potent neutralizing human monoclonal antibodies against cytomegalovirus infection from immune B cells

  10B7 1F7 26A1 8C10
Donor CMV5 CMV5 CMV7 CMV7
Doubling Time (days) 4 4 4 4
IgG Subclass IgG1 IgG1 IgG1 IgG2
BEIA-CMV a - - + +
gB ELISA + - - +
gH ELISA - + - -
HELF binding b - - - -
HUVEC binding b - - - -
HCMV neutralization c 64.72 ± 11.34 47.23 ± 2.98 86.34 ± 8.15 52.3 ± 13.34
  1. a. Antibodies were tested using the BEIA CMV ELISA assay
  2. b. Antibodies were tested for binding to uninfected HELF and HUVEC by immunohistochemistry.
  3. c. Antibodies were tested using the HCMV microneutralization assay, with the HCMV AD169 strain and HELF. Results are expressed as % of inhibition of virus infectivity. Data represent the mean ± s.d. of 3–4 experiments.