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Figure 4 | BMC Biotechnology

Figure 4

From: Induction of multiple pleiotropic drug resistance genes in yeast engineered to produce an increased level of anti-malarial drug precursor, artemisinic acid

Figure 4

Reverse-transcriptase PCR analysis of selected genes involved in multiple pleiotropic drug resistance. (A) Semi-quantitative RT-PCR conducted with RNA extracted after 24 hr of induction from EPY307 (artemisinic acid producer) and EPY309 (amorphadiene producer). Number of PCR Cycles used: ACT1: 23; PDR5, PDR15, SNQ2: 24; YOR1:27; PDR1:28; PDR3, PDR12:30. (B) Quantitative real-time RT-PCR conducted with samples harvested at 24 and 96 h post-induction.

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BMC Biotechnology

ISSN: 1472-6750

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