Amorphadiene production in the engineered yeast strain. (A) Plasmid harboring ADS under control of the Gal1 promoter used to produce amorphadiene in EPY300. Total (B) and specific (C) amorphadiene production from EPY300 expressing ADS on either pRS425-leu2 or pRS425-leu2d cultured in either CSM or YPG medium. Circles and diamonds indicate the leu2d and leu2 selection markers, respectively. Unfilled symbols indicate selective, minimal medium (CSM), and filled symbols indicate non-selective, rich medium (YPG). (D) Plasmid stabilities of yeast harboring pRS425-leu2::ADS or pRS425-leu2d::ADS grown in either CSM or YPG medium at 24, 72, and 144 hr post-induction. Data are mean ± standard deviation from three replicate experiments.