Figure 2

Optimization of the different Renilla luciferase reporter constructs. Zebrafish one to eight-cell embryos were microinjected with 30 ng EcLPS (A, C) or 6.5 ng VaDNA (B, C) and NF-κB::Luc together with the pRL-CMV, pRL-TK, pRL-SV40 or pRL-EF1α (10:1) reporter vectors. Twenty-four hours after microinjection, the firefly and Renilla luciferase activity was measured using the Dual-Luciferase Reporter Assay System. The results are presented as the Renilla luciferase activity (A, B) or as the normalized luciferase activity (firefly/Renilla) (C). Each bar represents the mean ± S.E. of ten replicate samples and the data are representative of three independent experiments. The asterisk denotes statistically significant differences between the indicated samples.