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Figure 1 | BMC Biotechnology

Figure 1

From: Application of the dual-luciferase reporter assay to the analysis of promoter activity in Zebrafish embryos

Figure 1

Analysis of zfTERT promoter activity in whole zebrafish embryos. Zebrafish one to eight-cell embryos were microinjected with plessEGFPLuc, zfpTERT(1 Kb)-EGFPLuc or zfpTERT(3 Kb)-EGFPLuc and the pRL-CMV (10:1) reporter vectors. Twenty-four hours after microinjection, the firefly and Renilla luciferase activity was measured using the Dual-Luciferase Reporter Assay System. The results are expressed as the mean ± S.E. of normalized luciferase activity relative to plessEGFPLuc injected embryos. *p < 0.05 vs. plessEGFPLuc. †p < 0.05 vs. zfpTERT(1 Kb)-EGFPLuc.

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BMC Biotechnology

ISSN: 1472-6750

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