Figure 2
From: Spherezymes: A novel structured self-immobilisation enzyme technology
Lipase spherezymes immobilisation method. Lipase (a) in aqueous medium (left) is added to an excess of a hydrophobic solvent (right), forming a water-in-oil emulsion (b), wherein the lipase migrates to the phase boundary and orientates the hydrophobic face associated with the active site towards the hydrophobic layer. The enzyme is subsequently cross-linked (grey crescents) to make the structure permanent (c) and the solvent removed (d).