SEM images of a laser-manipulated and a control larva reared to 2 dpf. (a, b) Whole body and antero-lateral views of a laser-manipulated larva at 2 dpf. (c, d) Same larva as in (a, b). Key developmental features that are illustrated are the yolk sac (YS), yolk sac extension (YSE), dorsal fin (DF), caudal fin (CF) and the olfactory pit (OP). (e, f) Whole body and antero-lateral views of a control larva at 2 dpf. (g, h) Same larva as in (e, f). The same developmental features observed in (c, d) were also seen in (g, h). Scale bars for (a, c, e, g) represent 200 μm and for (b, d, f, h) 100 μm.