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Table 1 Cat-activity of tested MG-cat-(i) and MG-cat-(i+j) strains. The results were averaged over three independently grown cultures for each clone; the scatter was 5–15%.

From: Dual-In/Out strategy for genes integration into bacterial chromosome: a novel approach to step-by-step construction of plasmid-less marker-less recombinant E. coli strains with predesigned genome structure

strain MG-cat-(i) IS element Activity, nmol/minxmg
MG-cat-(1) IS 5.7 180 ± 15
MG-cat-(2) IS 5.8 180 ± 15
MG-cat-(3) IS 5.9 240 ± 30
MG-cat-(4) IS 5.10 280 ± 15
MG-cat-(5) IS 5.11 270 ± 15
MG-cat-(6) native φ80-attB 200 ± 30
MG- cat- (i+j)   
MG-cat-(1+2) IS 5.7 IS 5.8 330 ± 15
MG-cat-(1+3) IS 5.7 IS 5.9 420 ± 30
MG-cat-(2+3) IS 5.8 IS 5.9 420 ± 30
MG-cat-(1+2+3) IS 5.7 IS 5.8 IS 5.9 520 ± 15
MG-cat-(4+5) IS 5.10 IS 5.11 500 ± 30
MG-cat-(4+6) IS 5.10 native φ80-attB 370 ± 15
MG-cat-(5+6) IS 5.11 native φ80-attB 400 ± 15
MG-cat-(4+5+6) IS 5.10 IS 5.11 native φ80-attB 540 ± 30