Table 1 Cat-activity of tested MG-cat-(i) and MG-cat-(i+j) strains. The results were averaged over three independently grown cultures for each clone; the scatter was 5–15%.
strain MG-cat-(i) | IS element | Activity, nmol/minxmg |
|---|---|---|
MG-cat-(1) | IS 5.7 | 180 ± 15 |
MG-cat-(2) | IS 5.8 | 180 ± 15 |
MG-cat-(3) | IS 5.9 | 240 ± 30 |
MG-cat-(4) | IS 5.10 | 280 ± 15 |
MG-cat-(5) | IS 5.11 | 270 ± 15 |
MG-cat-(6) | native φ80-attB | 200 ± 30 |
MG- cat- (i+j) | Â | Â |
MG-cat-(1+2) | IS 5.7 IS 5.8 | 330 ± 15 |
MG-cat-(1+3) | IS 5.7 IS 5.9 | 420 ± 30 |
MG-cat-(2+3) | IS 5.8 IS 5.9 | 420 ± 30 |
MG-cat-(1+2+3) | IS 5.7 IS 5.8 IS 5.9 | 520 ± 15 |
MG-cat-(4+5) | IS 5.10 IS 5.11 | 500 ± 30 |
MG-cat-(4+6) | IS 5.10 native φ80-attB | 370 ± 15 |
MG-cat-(5+6) | IS 5.11 native φ80-attB | 400 ± 15 |
MG-cat-(4+5+6) | IS 5.10 IS 5.11 native φ80-attB | 540 ± 30 |