Figure 3From: Dual-In/Out strategy for genes integration into bacterial chromosome: a novel approach to step-by-step construction of plasmid-less marker-less recombinant E. coli strains with predesigned genome structure Integration of plasmid pAH162-λ attL -Tc R -λ attR -Cm R into MG1655-derived strains with different location of φ80- attB . Back to article page