Figure 2
From: Genomic insertion of lentiviral DNA circles directed by the yeast Flp recombinase
Blockage of the normal viral integration machinery increases the formation of LV DNA circles in transduced cells. HEK-SB/FGIP cells were infected with integration-proficient or integration-deficient lentiviral vectors. Hirt DNA was harvested 24 hours post-transduction and subsequently used for Q-PCR analysis to determine the amount of linear and circular lentiviral DNA. Transductions were performed in triplicates and presented as mean value + SD.