DGC | RS+DGC | DGC+MCS | DGC+FACS | |
---|---|---|---|---|
Purity | ca. 70% a | ca. 93% a | > 98% d | 90 – 95% f |
CD5+/CD19+ | CD5+/CD19+ | > 93% e | CD5+/CD19+ | |
CD19+ | ||||
86% (± 14%) b | ||||
CD19+ | ||||
90% (± 7%) c | ||||
CD5+/CD19+/CD23+ | ||||
Time of procedure | < 60 mins. | < 90 mins. | > 120 mins (increasing with sample volume) | > 120 mins (increasing with sample volume) |
Selection method | negative | negative | positive | positive |
Limitations | highly depending on WBC count | dependent on WBC count when < 20 × 106 cells/ml PB | long process time and expensive | long process time and very expensive |
cost | low | medium | medium-high | high |
Required Infra-structure | centrifuge | centrifuge | centrifuge + MCS Sorter | centrifuge + FACS sorter |