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Table 1 Comparison of the discussed four different enrichment approaches

From: Negative selection of chronic lymphocytic leukaemia cells using a bifunctional rosette-based antibody cocktail

  DGC RS+DGC DGC+MCS DGC+FACS
Purity ca. 70% a ca. 93% a > 98% d 90 – 95% f
  CD5+/CD19+ CD5+/CD19+ > 93% e CD5+/CD19+
    CD19+  
  86% (± 14%) b    
  CD19+    
  90% (± 7%) c    
  CD5+/CD19+/CD23+    
Time of procedure < 60 mins. < 90 mins. > 120 mins (increasing with sample volume) > 120 mins (increasing with sample volume)
Selection method negative negative positive positive
Limitations highly depending on WBC count dependent on WBC count when < 20 × 106 cells/ml PB long process time and expensive long process time and very expensive
cost low medium medium-high high
Required Infra-structure centrifuge centrifuge centrifuge + MCS Sorter centrifuge + FACS sorter
  1. DGC: density gradient centrifugation, RS+DGC: RosetteSep incubation prior to DGC, DGC+MCS: DGC and subsequent magnetic cell sorting, DGC+FACS: DGC and subsequent fluorescent activated cell sorting.
  2. a average purity experienced in our study
  3. b purity reported by Haslinger et al., 2004
  4. c purity reported by Falt et al., 2005
  5. d purity reported by Rosenwald et al., 2004 and Wiestner et al., 2003
  6. e Huttman et al., 2006
  7. f purity reported by Stankovic et al., 2004
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