Figure 4

In vitro synthesis of the fusion protein FG. A. Western blot analyses (anti-Fluc) of the in vitro synthesis reactions (at 60 min.) of Fluc, FG and F-SH in different systems. The full-length proteins, indicated by arrows, were confirmed by using the purified proteins of Fluc (purified Fluc) and FG (purified FG). B. The Fluc activities were determined for the Fluc and FG templates after 60 min. of the in vitro synthesis reactions in different systems. The data are presented as the relative activities with the activity of Fluc in S30 at 60 min being 100. C. Western blot analyses of the time courses of the in vitro synthesis reactions of FG in S30+RRL and RRL using anti-Fluc and anti-GFP to probe the translation products. The positions of the full-length FG and Fluc, indicated by arrows, were confirmed by the purified FG (purified FG) and the in vitro synthesis reaction of Fluc (FLuc control). The molecular weights (kDa) are indicated at the left.