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Figure 6 | BMC Biotechnology

Figure 6

From: Successful elimination of non-neural cells and unachievable elimination of glial cells by means of commonly used cell culture manipulations during differentiation of GFAP and SOX2 positive neural progenitors (NHA) to neuronal cells

Figure 6

Subcloning method revealed undifferentiated NHA cells as non-committed homogenous cell population. Immunocytochemistry results show: co-expression of GFAP and MAP2 in undifferentiated NHA cells passage 0 seeded at a very low density (5 000 cells/cm2) (a); equal "non-colonial" distribution of cells representing phenotype [MAP2+, CD44-] and phenotype [CD44+, MAP2-] obtained as a result of neural differentiation of NHA cells which previously reached confluence in medium with serum (b); ability of NHA cells to form colonies obtained as a result of neural differentiation of non-confluent cells (c).

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BMC Biotechnology

ISSN: 1472-6750

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