Figure 7

Bad/14-3-3 split-TEV assays in HEK293 and NIH-3T3 cells using a fluorescent reporter. (a-l) FACS analyses of HEK293 (a-f) and NIH-3T3 (g-l) cells that were co-transfected with the GV2-ER and G5-EYFPnuc reporter and the indicated expression plasmids (Mock indicates empty expression vector pcDNA3). (a-e, g-l) Representative histograms of replicate analyses with the YFP-fluorescence plotted against the PI-fluorescence intensities. Q1 gated cells display increased YFP fluorescence, Q2 gated cells are YFP/PI double-positive cells, Q3 are YFP/PI negative cell populations and Q4 represent PI positive cells. (f, l) Quantitative analysis of Q1 gated events from all replicates (n = 3) given as number of YFP-positive per 10000 (f, HEK293) or 5000 (l, NIH-3T3) cells, respectively.