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Figure 5 | BMC Biotechnology

Figure 5

From: Analysis of transient phosphorylation-dependent protein-protein interactions in living mammalian cells using split-TEV

Figure 5

Bad/14-3-3 split-TEV assays in primary cultured neurons. (a) Luciferase reporter assay quantifying the regulated interaction of murine Bad and 14-3-3 isoforms in murine primary cultured cortical neurons. Cells were transfected with the indicated constructs and LucER as reporter, n = 6. (b) Renilla luciferase readings in primary cultured neurons. Measurements were obtained from the same experiment as shown in (a). The values are given as the ratio of the mean value of the Renilla luciferase readings and the number of seeded cells per well (60000).

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BMC Biotechnology

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