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Figure 2 | BMC Biotechnology

Figure 2

From: Analysis of transient phosphorylation-dependent protein-protein interactions in living mammalian cells using split-TEV

Figure 2

Homo- and heterodimerization of 14-3-3 isoforms. (a) Schematic representation of a 14-3-3 split-TEV assay. 14-3-3 isoforms were fused to N-TEV and C-TEV fragments. Upon homo- or heterodimeric interaction the TEV protease activity is reconstituted. (b) Murine 14-3-3 isoforms ε and ζ form homo- and heterodimers. Luciferase readings also verify the flexible usage of TEV fragments. GCN4cc domains were used as negative controls. NIH-3T3 cells were transiently transfected with the constructs as indicated. RLUs, relative luciferase units, n = 6.

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BMC Biotechnology

ISSN: 1472-6750

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