Figure 1
![Figure 1](http://media.springernature.com/full/springer-static/image/art%3A10.1186%2F1472-6750-8-51/MediaObjects/12896_2008_Article_320_Fig1_HTML.jpg)
Sequence and illustration of pTBSG and its derivatives. Following the His tag at the very N terminus, different fusion partners (KSI, GST and MSP) were inserted between the restriction enzyme sites for SpeI and KpnI. A tobacco etch virus protease (TEV) cleavage site was inserted between the fusion partner protein and the target protein which was inserted at the SspI site through LIC.