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Figure 1 | BMC Biotechnology

Figure 1

From: Construction of a series of vectors for high throughput cloning and expression screening of membrane proteins from Mycobacterium tuberculosis

Figure 1

Sequence and illustration of pTBSG and its derivatives. Following the His tag at the very N terminus, different fusion partners (KSI, GST and MSP) were inserted between the restriction enzyme sites for SpeI and KpnI. A tobacco etch virus protease (TEV) cleavage site was inserted between the fusion partner protein and the target protein which was inserted at the SspI site through LIC.

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BMC Biotechnology

ISSN: 1472-6750

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