Figure 5

Inhibition of CD147 surface expression on 293A cells by M6-1B9 intrabody. 293A cells were transduced with recombinant adenovirus harboring scFv-M6-1B9 or scFv-SVV3. Cell surface staining of CD147 on A untransduced and B scFv-M6-1B9 or C scFv-SVV3 transduced cells was performed using CD147 mAb, M6-1B9 (bold lines) or irrelevant isotype matched mAb (dashed lines). PE-conjugated F(ab')2 fragment of sheep anti-mouse immunoglobulins antibody were used as a secondary antibody. The percentage (%) of CD147 positive cells was indicated.