Table 4 cDNA targets and PCR primers.
Acronym | Locus Tag | 5' Primer | 3' Primer | Size (bp) | Amplicon%GC |
LAM | LAMCG | K7B: CTGCTGGCCGGAACTAATGAATTTATTGGT | K12: ATGCCACGATGCCTCATCACTGTTG | 150 | 50.0% |
| Â | Â | K23: TCTGGCTACGTCCTGATGCAGGG | K30 : TTCCTGAGACAATACAGCACGACCGCTG | 150 | Â |
CHS | AT5G13930 | K3: CACGACAGGAGAAGGGTTGGAGT | K4: GCGTAGGTAGGTAGGCAGATAGAAGGC | 132 | 53.0% |
SE | AT2G27100 | K3: AGCTACCAAGACCTAGATGCTCCAGAG | K2: CGGGATCTCTCTAGCCCTGTCTTGT | 88 | 48.9% |
PHB | AT2G34710 | K3: TCAAGCATGGGAAGGATGGTATCTTACGA | K2: AAGCTAAGCAGTGGTTTGATTCATCGTCTTCA | 85 | 45.9% |
EMF2 | AT5G51230 | K3: GGAGAGTGTTTATGGTGAAACTGTGGAACC | K2: GGAGCTGTTCGAGAAAGGTATTACAGTTGTTC | 87 | 47.1% |
NPR1 | AT1G64280 | K1: GAAATTCGTCCCTGACAGATTCGACTTCT | K4: CACTAAGAGGCAAGAGTCTCACCGAC | 110 | 48.2% |
ANT | AT4G37750 | K3: CGGATCAAATATGGGCGGAAATATGAGTCCTT | K4: TCAAGAATCAGCCCAAGCAGCGAAAAC | 118 | 48.3% |
FLF/FLC | AT5G10140 | K3: CACCTGCTGGACAAATCTCCGACAA | K4: TCATAATTATATATGTTTTGGATTTTGATTTCAACCGCCG | 107 | 40.2% |
HAP3C | AT2G37060 | K3: GATTACGCCGCCCAGCTCAAGA | K4: GCGATGGAAGAATTTTAATTATCTGGCGAGGATTTAGG | 122 | 45.9% |
ABI3 | AT3G24650 | K3: CCCACCGTCGTCAGCAGCTAC | K4: TCATTTAACAGTTTGAGAAGTTGGTGAAGCGAC | 115 | 47.0% |
AP3 | AT3G54340 | K3: CCATGGCCTTCATGCACCCTCT | K4: ACTAAATAATTAGATAGACAATGATGGCACCAGCAAACC | 110 | 40.0% |
LEC2 | AT1G28300 | K3: TCCTCAATGACACCTGAGGATCACG | K4: GGGTTATGAACTTGAGAACTTCCACCACCATA | 113 | 48.7% |