Figure 4

Optimum temperature (A) and thermostability (B) of the GBSV1-NSN nuclease. The nuclease activity of GBSV1-NSN was assayed at various temperatures ranging from 20°C to 80°C. To evaluate the thermostability, the residual enzymatic activities were monitored after incubation of the enzyme solutions (295 μg/ml) in the absence of herring dsDNA (100 μg/ml) at 40, 50, 60 or 70°C (pH 7.5) for 15, 40, 60, 90 or 130 min. The nuclease activity assay was conducted using 100 μg/ml of herring dsDNA as substrate. Each point represented the mean of triplicate assays and the error bars showed the standard deviations.