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Figure 4 | BMC Biotechnology

Figure 4

From: Comparison of the developmental potential of 2-week-old preantral follicles derived from vitrified ovarian tissue slices, vitrified whole ovaries and vitrified/transplanted newborn mouse ovaries using the metal surface method

Figure 4

Growth rate of preantral follicles derived from frozen-thawed and transplanted newborn mouse ovary. The granulosa cells outgrowth rate of preantral follicle after isolation from fresh transplanted ovaries (circle, n = 28) and frozen-thawed transplanted ovaries (square, n = 31) during culture for 10 days in IVG medium. These ovaries were collected from newborn mice and immediately frozen-thawed with the vitrification method and then transplanted to the renal capsule of recipient mice for 14 days. The fresh ovaries were collected from newborn mice and only transplanted without freezing. No significant difference was found in the diameter of the granulosa cell outgrowth of follicles after 10 day of culture.

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