Figure 3
Dose-response profile of dystrophin expression after intramuscular injections of CG-PEI2K(PEG5K) 10 -ESO polyplexes. The TA muscles of mdx mice were given 3 weekly intramuscular injections of CG-PEI2K(PEG5K)10 copolymers complexed with 1, 5, 10, or 20 μg of ESO per injection (3, 15, 30, and 60 μg total) and harvested 3 wks after the first injection. Images show dystrophin immunolabeling (Hoechst dye counterstained) and H&E staining of serial transverse sections from TA muscles for each of the 4 polyplex doses.