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Figure 3 | BMC Biotechnology

Figure 3

From: Improved production of human type II procollagen in the yeast Pichia pastoris in shake flasks by a wireless-controlled fed-batch system

Figure 3

P. pastoris cultivation in shake flasks with methanol feeding without (A, B) or with (C, D) manual pH adjustment. Cultivations were performed in two phases: initial batch phase in BMG-medium and fed-batch in BMM-medium. Dissolved oxygen (pO2) and pH were measured with a wireless measuring system, cell growth was followed by measurement of the OD600. Vertical lines represent methanol feeding points in A and C or start of methanol feed in B and D.

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BMC Biotechnology

ISSN: 1472-6750

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