Figure 3

Comparison of IPNS protein produced by H. polymorpha IPNS 4.2 cultivated at various temperatures. Western blot showing the relative amounts of IPNS protein produced by mid-exponentially grown H. polymorpha IPNS 4.2 cells cultivated at 37°C, 30°C and 25°C (lanes 3, 4 and 5, respectively). As controls, H. polymorpha WT cells, cultivated on methanol at 25°C (lane 2), and P. chrysogenum cells, cultivated for 40 h on penicillin production media supplemented with phenylacetic acid (lane 1), are included. These demonstrate the specificity of the α-IPNS antiserum.