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Table 4 Percentage and fold-amplification of specific DNA determined by real-time PCR

From: Amplification of multiple genomic loci from single cells isolated by laser micro-dissection of tissues

 

Real-time PCR concentration (ng/μl)

    

Cell

X1

X6

X11

SRY

ND3

Nanodrop (ng/μl)

Specific genomic (%)

Specific mitochondrial (%)

Fold-amplification (copies)

1

133

22.8

2.36

78.0

81.8

112

52.7

73

1.1×106

2

27.1

10.7

7.1

41.6

293

51

42.4

574

4.3×105

3

25.6

33.8

4.5

48.1

193

58

48.2

332

5.5×105

4

21.8

23.8

17.9

75.1

151

77

45.0

196

6.9×105

5

25.9

40.4

2.3

67.5

266

56

60.7

475

6.7×105

6

22.2

6.14

13.9

36.7

55.5

46

42.8

120

3.9×105

7

30.4

67.5

1.8

153

1006

144

43.8

698

1.2×106

8

8.06

12.6

2.7

13.5

882

50

18.4

1764

3.8×105

Cells 1–8 (mean ± std)

44.2 ± 12

529 ± 545

6.9×105 ± 3.4×105