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Table 3 Mean amplification and dropout rates for cells

From: Amplification of multiple genomic loci from single cells isolated by laser micro-dissection of tissues

 

Singleplex

Multiplex

 

Amp/L

Amp/A

ADO

Amp/L

Amp/A

ADO

Fresh

70.3 ± 15.5

63.5 ± 16.3

64.2 ± 27.6

66.9 ± 12.9

45.4 ± 10.5

71.9 ± 13.9

Pre-stored

36.5 ± 15.5

32.9 ± 14.2

75.5 ± 28.4

40.4 ± 12.6

24.7 ± 7.5

85.3 ± 3.8

P score

2.1×10-5

3.6×10-5

0.48

2.1×10-5

2.1× -5

3.7×10-4

  1. Each result represents mean percentage ± standard deviation of the corresponding distribution that is presented in Additional file 2. For fresh cells, mean amplification values differ slightly from corresponding rates in table 1, because absence of uncounted samples (due to positive signals in the corresponding negative controls) tend to affect the average slightly differently in horizontal vs. vertical calculation (i.e. when calculated per cell vs. per locus).
  2. P scores represent Kolmogorov-Smirnov goodness-of-fit hypothesis test scores for comparisons between the respective distributions.