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Figure 4 | BMC Biotechnology

Figure 4

From: Simplified approaches for the development of an ELISA to detect circulating autoantibodies to p53 in cancer patients

Figure 4

Representative results of serum p53 autoantibodies of lung cancer patients detected by immunoblotting. PVDF membrane containing 1 μg/strip of the purified (His)6-p53 fusion protein was incubated with 1:5,000 p53 mAb DO7 as positive control, 1:200 diluted sera from lung cancer patients, respectively. Lane PC, positive control: probing with p53 mAb DO7, lane A-I, probing with serum from lung cancer patients.

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