Figure 4From: Simplified approaches for the development of an ELISA to detect circulating autoantibodies to p53 in cancer patientsRepresentative results of serum p53 autoantibodies of lung cancer patients detected by immunoblotting. PVDF membrane containing 1 μg/strip of the purified (His)6-p53 fusion protein was incubated with 1:5,000 p53 mAb DO7 as positive control, 1:200 diluted sera from lung cancer patients, respectively. Lane PC, positive control: probing with p53 mAb DO7, lane A-I, probing with serum from lung cancer patients.Back to article page