Figure 1

Western blot analysis of (His)6-p53 fusion protein (A) and biotinylated p53-BCCP fusion protein (B) expressed in E. coli BL21(DE3)pLysS strain and Origami strain, respectively. Cell lysate from E. coli BL21(DE3)pLysS strain transformed with pET15b empty vector was also analyzed as a negative control for analysis of (His)6-p53 fusion protein. E.coli Origami strain transformed with pAK400 harbouring CD147 encoding DNA was analysed as a negative control for analysis of biotinylated p53-BCCP fusion protein. Fifteen micrograms of crude cell lysates were electrophoresed and electroblotted onto PVDF membranes. The PVDF membrane was incubated with either mouse anti-p53 mAb (DO7), mouse anti-histidine mAb or mouse anti-biotin mAb, respectively. Lane 1, bacterial cell lysate from negative controls; lane 2, lysate from p53 expressing cells