Figure 4
Results of SDS-PAGE and Western blot analysis of RpoS-fusion and fusion-free G-CSF. -SDS-PAGE analysis (lane M-3): lane M, molecular markers; lane 1, supernatant of recombinant E. coli cell lysates containing recombinant (His)6-RpoS-D4K-G-CSF, which was loaded onto ProBond resin (Ni+2) column for metal affinity purification; lane 2, soluble fraction of enterokinase(EK)-digested product of (His)6-RpoS-D4K-G-CSF, containing RpoS and fusion-free G-CSF (indicated by an arrow); lane 3, purified soluble fusion-free G-SCF. -Western blot analysis (lane 4): result of immunoblotting analysis of purified soluble fusion-free G-SCF (loaded onto lane 3).