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Table 3 Characterization of the fermentation process for the secretion of recombinant Bm86, Bd86 and Ba86.

From: Expression of recombinant Rhipicephalus (Boophilus) microplus, R. annulatus and R. decoloratus Bm86 orthologs as secreted proteins in Pichia pastoris

Recombinant strain Mut phenotype O.D. 600 nm before induction μmax in glycerol (h-1)a μmax in methanol (h-1)b Total protein concentration (mg·L-1)c Recombinant protein
       Concentration (mg·L-1)c Purity (%)c Productivity (mg·L-1·h-1)c
GS115Moz9-2 MutS 115 0.181 0.005 274 150 55 2.1
KM71HDec8-1 MutS 125 0.182 0.002 194 110 57 1.5
X33pBaI 3 MutS 125 0.178 0.003 170 112 66 1.6
  1. aThe maximum growth rate (μmax) was determined during the exponential growth phase on glycerol in batch and fedbatch modes.
  2. bThe maximum growth rate (μmax) was determined during the exponential growth phase on methanol (first 20–24 hrs after induction).
  3. cDetermined in the culture medium 72 hrs after induction with methanol using the Bradford method with BSA as standard [49] and the Experion semiautomated electrophoresis system (Bio-Rad, Hercules, CA, USA).