Quantification of PRL/Stat5 signaling with a novel pGL4-CISH reporter

BMC Biotechnology

Table 1 Comparison of Reporter Responses to PRL in T47D cells

Reporters	DNA source	Stat5 binding sites	Sensitivity to PRL	References
pGL4-CISH	human	4	+ + +	this work
LHRE-TK-luc	synthetic tandems	6	+ +	[17]
pGL4-CCND300	human	1	+	this work
Cyclin D1Δ-944	human	2	+	[18]
Beta-casein-2300	rat	2	+ +	[21]

(+ + +, most sensitive; + +, sensitive; +, less sensitive in terms of reporter readout)

ISSN: 1472-6750