Skip to main content
Figure 6 | BMC Biotechnology

Figure 6

From: Improved RNA quality and TaqMan®Pre-amplification method (PreAmp) to enhance expression analysis from formalin fixed paraffin embedded (FFPE) materials

Figure 6

Comparison of TaqMan® real time PCR with or without PreAmp using CDKN1B assay on extracts produced by different extraction protocols. (1 and 2 = Stratagene extracts; 3 and 4 = Ambion extracts; 5 and 6 = Gentra extracts; 7 and 8 = Trizol extracts; * = No amplification was achieved from FFPE) ΔCT = CT (no_PreAmp) - CT (with_PreAmp). The Theoretical ΔCT was 5.68 [= Log2(1024 ng/20 ng)] which was calculated based on an input of 1024 ng of cDNA for the TaqMan® real time PCR component of pre-amplification process and 20 ng of cDNA for the TaqMan® without pre-amplification. Ideally, ΔCT is in a range between Theoretical ΔCT +/- 1 which was achieved in this experiment indicating there is no bias in the PreAmp.

Back to article page

BMC Biotechnology

ISSN: 1472-6750

Contact us