Figure 4

Ad/GFP _DiSTRES demonstrates specificity for prostate cells, decreased activity in non-prostate control cells, and improves upon earlier vector designs. GFP fluorescence of (a) LNCaP cells and (b) U251MG cells. LNCaP and U251MG cells were seeded at 1.25 × 10⁴ cells/well of a 96-well plate and infected with Ad/GFP_TET, Ad/GFP_PS/TR, Ad/GFP_PFLPS, Ad/GFP_DiSTRES, or Ad/C.LacZ (negative control) in the presence of 30 nM DHT at MOI 1, 10, 100, or 1000. 2 days post-infection, cell lysates were assayed for GFP fluorescence. RFU: relative fluorescence units. MOI values were based on IU/ml.